Contents

Biological Clocks in Mosquitoes
Laboratory recordings of flight activity of Aedes (Stegomyia) aegypti ssp aegypti, type-form, LSHTM strain

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(i) Ae. aegypti ssp aegypti, type-form, LSHTM strain

Experimental material
The adults used in the experiments were reared under LD 12:12 from a colony established at Brunel University in 1966, using eggs obtained from the London School of Hygiene and Tropical Medicine. The source colony had originated from West Africa (latitude about 10°N) and had been maintained since 1926.

Experimental regimes
LD 4:20, nine females, studied from 5-6 days post-emergence (4-5 May 1968). Recorded in LD 12:12 on day three then light-on delayed 8h to give LD 4:20 for days four to eight.
LD 8:16, eight females, studied from 6-7 days post-emergence (21-22 November 1967). Recorded in LD 12:12 on day two then light-on delayed 4h to give LD 8:16 for days three to seven.
LD 12:12, six females, studied from 1-2 days post-emergence (20-21 December 1967). Recorded for days six to nine.
LD 14:10 to LD 18:6, nine females, studied from 5-6 days post-emergence (23-24 October 1968). Recorded in LD 14:10 for days two to four then light-on advanced 4h to give LD 18:6 for days five and six.
LD 16:8, eight females, studied from 6-7 days post-emergence (14-15 November 1967). Recorded in LD 12:12 on day three then light-on advanced 4h to give LD 16:8 for days four to seven.
LD 20:4, nine females, studied from 5-6 days post-emergence (26-27 April 1968). Recorded in LD 12:12 on day three then light-on advanced 8h to give LD 20:4 for days four to seven.

Results
The activity patterns are shown in Figure A1 below. The pattern in all seven LD regimes is dominated by the E' peak. Very little activity follows in the subjective night, and there is a small, variable, M peak which is rather more pronounced when L = 16h or longer. It is clear that when L > 16h or more the E' peak falls progressively earlier as L increases, coming some 13-14h after light-on.

Figure A1
Aedes aegypti

Contents
©1998, 2010 - Brian Taylor CBiol FSB FRES
11, Grazingfield, Wilford, Nottingham, NG11 7FN, U.K.

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