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Biological Clocks in Mosquitoes
Laboratory recordings of flight activity of Aedes (Stegomyia) aegypti ssp aegypti type-form Ilobi strain
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(ii) Ae. aegypti ssp aegypti type-form Ilobi strain

Experimental material
The adults were reared, under LD 12:12, from eggs obtained from the East African Virus Research Institute, Uganda, where a colony originating from Ilobi, near Lagos, south-western Nigeria (latitude 6°20' N) had been maintained for about ten years. This colony was that used for much of the extensive research on oviposition and sugar-feeding (see Gillett, 1962).

Experimental regimes
LD 8:16 to LD 4:20, six females, studied from 3-4 days post-emergence (22-23 November 1968). Recorded in LD 8:16 for days six to eight then light-on delayed 4h to give LD 4:20 for days nine to eleven.
LD 12:12 to LD 16:8, six females, studied from 3-4 days post-emergence (22-23 November 1968). Recorded in LD 12:12 for days six to eight then light-on advanced 4h to give LD 16:8 for days nine to eleven.
LD 20:4, six females, studied from 3-4 days post-emergence (22-23 November 1968). Recorded for days eight to twelve.

Results
The activity patterns are shown in Figure A2 below.
The pattern in all five LD regimes is dominated by the E' peak. Very little activity follows in the subjective night and there is a variable M peak. This is most pronounced in LD 16:8 (the first cycle shown is when the advancing of light-on from LD 12:12 took place, so the large M may be due to the transient effect of phase resetting). It is clear that when L > 16h the E' peak falls progressively earlier as L increases, coming some 13-14h after light-on.

Figure A2
Aedes aegypti Ilobi form

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©1998, 2010 - Brian Taylor CBiol FSB FRES
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