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Biological Clocks in Mosquitoes
Laboratory recordings of flight activity of Aedes (Stegomyia) aegypti ssp formosus wild-form, Bwamba strain

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(iii) Ae. aegypti ssp formosus wild-form, Bwamba strain

Experimental material
The adults were reared, under LD 12:12, from eggs obtained from the East African Virus Research Institute, Uganda, where a colony originating from Bwamba, western Uganda (latitude 0°45'N) had been maintained for about six months.

Experimental regimes
LD 4:20, five females, studied from 5-6 days post-emergence (5-6 December 1968) and recorded for days eight to ten.
LD 8:16, nine females, studied from 3-4 days post-emergence (31 October 1968) and recorded for days seven and eight.
LD 12:12 to LD 16:8, nine females, studied from 3-4 days post-emergence (31 October 1968). Recorded in LD 12:12 for days six to eight then light-on advanced 4h to give LD 16:8 for days nine to eleven.
LD 20:4, six females, studied from 3-4 days post-emergence (31 October 1968) and recorded for days six to eight.

Results
The activity patterns are shown in Figure A3 below.
The pattern in all five LD regimes is dominated by the E' peak. There is some activity in the subjective night, most obviously in LD 4:20 with a minor peak 3-4h after light-off. There is a clear M peak, which becomes broader when L > 12h, peaking at 2-2.5 h after light-on in LD 20:4. It is clear that when L > 14h the E' peak falls progressively earlier as L increases, coming some 14-15h after light-on.

Figure A3
Aedes aegypti Bwamba

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©1998, 2010 - Brian Taylor CBiol FSB FRES
11, Grazingfield, Wilford, Nottingham, NG11 7FN, U.K.

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